Inhibition of rapamycin on proliferation of human acute promyelocytic leukemia HL60 cells / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 2002-2005, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-860131
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To investigate the effect of rapamycin on proliferation, autophagy and apoptosis of human acute promyelocytic leukemia HL60 cells.METHODS:
The inhibitory effect of rapamycin on HL60 cell was detected by MTT assay. The expressions of LC3-II autophagy-related protein were determined by Western blot. The cell cycles were analyzed by flow cytometry (FCM). Apoptosis were detected by DNA agarose gel electrophoresis.RESULTS:
After treatment with rapamycin of 0, 1, 5, 10, 20, 40 nmol · L-1 for 24 h, proliferation of HL60 cells were inhibited in a dose-dependent manner (r=0.97, P < 0.05). Western-blot shows that the expression of LC3-II protein of each concentration group was higher than that of the control group (P < 0.01). Rapamycin has a role in promoting cell autophagy. Compared with control group, more cells were arrested at G0/G1 phases (P < 0.05) and fewer cells were at S phases (P < 0.05). Agarose gel electrophoresis shows no apoptosis DNA fragments.CONCLUSION:
In given concentration range, rapamycin can inhibit the proliferation of human acute promyelocytic leukemia HL60 cells by promoting autophagy rather than inducing apoptosis.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Document type:
Article