Determination of eight ginsenosides in Renshenshouwu capsules by HPLC-ELSD / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop an HPLC-ELSD method for simultaneous determination of the contents of eight ginsenosides in Renshenshouwu capsules (traditional Chinese medicines). METHODS: The analysis was performed on an Lltimate C18 column (4.6 mm × 250 mm, 5 μm) eluted with mobile phase consisting of acetonitrile and water in gradient mode at a flow rate of 1.0 mL · min-1. The column temperature was set at 30°C. The ELSD drift tube temperature was set at 100°C, and the gas flow rate was set at 2.5 L · min-1. RESULTS: Good linearity(r > 0.9995) was obtained in the range of 0.483-12.1 μg, 0.444-11.1 μg, 0.502-12.6 μg, 0.490-12.3 μg, 0.455 23.2 μg, 0.464-11.6 μg, 0.264-13.2 μg and 0.244-12.2 μg for ginsenoside Rg1, ginsenoside Re, gin-senoside Rb1, ginsenoside Rc, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rd and 20(S)-ginsenoside Rg3, respectively. The average recoveries of the eight compounds were 101.9%, 104.5%, 98.3%, 97.9%, 97.8%, 101.2%, 100.6% and 99.0% Respectively. CONCLUSION: The method is reliable, simple, precise, and can be used for the quality control of Renshenshouwu capsules.