Urine excretion of neopanaxadiol in rats by UPLC-MS/MS / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 636-639, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-860416
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To develop a UPLC-MS/MS method for the determination of neopanaxadiol (NPD) in rat urine samples, and to explore the excretion patterns of NPD in rats after oral administration.METHODS:
NPD was extracted from u-rine samples by liquid-liquid extraction. The concentration of NPD in urine was determined by UPLC-MS/MS and the cumulative excretion amount and excretion rate of NPD were calculated after a single oral administration of NPD at 100 mg · kg-1 to SD rats.RESULTS:
Excellent linearity was found between 80-1280 ng · mL-1. The intra-and inter-day RSDs of the QC samples were both below 15% and the extraction recoveries of NPD were higher than 80%. The cumulative excretion of unchanged NPD in urine within 96 h amounted to (0.0233±0.0356)% of the dose.CONCLUSION:
NPD is hardly eliminated through urine within 96 h in rats.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2013
Document type:
Article