Effect of berberine on the whole genome transcription of methicillin-resistant Staphylococcus aureus / 中华检验医学杂志

ABSTRACT

Objective:The work aims to analyze the effect of berberine on the transcriptome-feature of the methicillin-resistant Staphylococcus aureus (MRSA) USA300 by using transcriptome sequencing (RNA-seq methods) methods and to investigate the bacteriostatic mechanism of berberine on MRSA. Methods:Basic research. The standard strain USA300 was used in this study, which was a honorable gift from Dr. Lan Lefu in Shanghai Institute of medicine, Chinese Academy of Sciences in 2019. The differential gene expression profiles of berberine treated [1/2 MIC (64 mg/L) and 1/8 MIC (16 mg/L), respectively] and untreated USA300 were analyzed. The screening conditions for differentially expressed genes were set as P-Value<0.05, and the | Fold-change |>2. SPSS 20.0 was used to analyze the result. Results:Sequencing results showed that there were 754 differentially expressed genes in the high concentration group compared with the normal control group, of which the up-regulated genes were lukD, lukE, ssaA, etc., while the down-regulated genes were lukF-PV, lukS-PV, clfA, splB-splF, sspA,etc.. Only 19 genes were differentially expressed in the low concentration group compared with the normal control group, of which the up-regulated genes were speG, scpB, rpsQ,etc., while the down-regulated genes were beta,bccT, etc.Conclusions:In this study, the gene expression of USA300 transcriptome was studied to understand the differential gene expression after berberine treatment. It was speculated that berberine played a role in inhibiting MRSA by regulating the hydrolysis of bacterial cell wall, the expression of leucotoxin and other virulence factors.