Analysis of SCN1A gene variants among patients with Dravet syndrome / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 158-161, 2021.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-879545
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To analyze the clinical features and genetic variants in two patients with Dravet syndrome (DS).@*METHODS@#Peripheral blood samples of the children and their parents were collected for the extraction of genomic DNA and high-throughput sequencing. Suspected variants were confirmed by Sanger sequencing.@*RESULTS@#By high-throughput sequencing, the two children were found to respectively harbor a c.2135delC frameshifting variant in exon 12 and a c.1522G>T nonsense variant in exon 10 of the SCN1A gene. Both variants were predicted to be pathogenic by bioinformatic analysis. Based on the American College of Medical Genetics and Genomics standards and guidelines, the c.2135delC and c.1522G>A variants of the SCN1A gene were predicted to be pathogenic (PVS1+ PS2+ PM2+ PP3).@*CONCLUSION@#The variants of the SCN1A gene probably underlay the DS in the patients. Above finding has enriched the variant spectrum and enabled genetic counseling for their families.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pedigree
/
Spasms, Infantile
/
Epilepsies, Myoclonic
/
Genomics
/
Mutation
Type of study:
Practice guideline
/
Prognostic study
Limits:
Humans
/
Infant
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2021
Document type:
Article