Establishing dose-response curve of chromosome aberration induced by in vitro X-ray irradiation in human peripheral blood / 中国职业医学

ABSTRACT

OBJECTIVE: To establish the dose-response curve of chromosome aberration induced by X-ray irradiation in human peripheral blood in vitro. METHODS: The median cubital vein blood of healthy male donors were collected and irradiated with X-ray at the dose of 0. 00-5. 00 Gy in vitro. The dose rate was 0. 8 mGy/s. The cells were cultured with colchicine and stained with routine Giemsa staining. The slices were blindly examined. The morphology of chromosomes were recorded as dicentric,multi-centric,dicentric plus rings or fragments( hereinafter referred to as dicentric + ring) in metaphase cells. The occurrence of dicentric + ring and the irradiation dose was used to create the dose-response curve.RESULTS: The results showed that the occurrence of dicentric + ring in abnormal cells increased with the increasing irradiation dose in the range of 0. 00-5. 00 Gy( P < 0. 01). The best fitting equation of 0. 00-1. 00 Gy is y = 23. 22 D2+4. 768 D-0. 018( P < 0. 01). The best fitting equation of 0. 50-5. 00 Gy is y = 34. 23 D-3. 072( P < 0. 01).CONCLUSION: The fitting degree of dose-response curve is good,which can be used as reference in laboratory to assess irradiation exposure dose.