Expression Change of Complement Regulator Genes of the Human Astrocytoma Cell Line after A beta1-42 and Interferon Gamma Administration
Journal of the Korean Geriatrics Society
; : 235-245, 2008.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-88280
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
We determined the changes of complement regulator gene expression in the amyloid-beta1-42(A beta1-42) and interferon-gamma(IFN-gamma)-stimulated human astrocytoma cell line.METHODS:
The human astrocytoma cell line, U373MG, was stimulated with IFN-gamma(62.5-1,000U/ml) in the presence or absence of aggregated A beta1-42(1-20micrometer) for 24 hours. Messenger RNA expression of C1 inhibitor(C1-INH), complement factor I(CFI), clusterin, vitronectin, decay accelerating factor(DAF), membrane cofactor protein(MCP), and CD59 was measured by quantitative real-time reverse transcriptase-PCR.RESULTS:
IFN-gamma(final concentration, 500U/ml) markedly increased the expression of mRNA for C1-INH in a time dependent fashion. A beta1-42(final concentration, 2micrometer) induced a slight increase in the expression of C1-INH. Messenger RNAs for CFI and clusterin were minimally increased, but other regulators were unchanged or decreased by either A beta1-42 or IFN-gamma. IFN-gamma overrode A beta1-42-induced mRNA expression of C1-INH when the cells were treated with these two reagents together.CONCLUSION:
Among the complement regulator genes in the human astrocytoma cell line, U373MG, only C1-INH was significantly up-regulated by IFN-gamma with or without A beta1-42 administration.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Astrocytoma
/
Complement System Proteins
/
RNA, Messenger
/
Cell Line
/
Genes, Regulator
/
Amyloid beta-Peptides
/
Interferons
/
Interferon-gamma
/
Complement Factor I
/
Vitronectin
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Geriatrics Society
Year:
2008
Document type:
Article