Blockade of Nitric Oxide Synthesis Further Diminishes Aquaporin Water Channels in Rat Kidney Subjected to Ischemia/reperfusion Injury / 대한신장학회잡지
Korean Journal of Nephrology
; : 7-12, 2006.
Article
in En
| WPRIM
| ID: wpr-89289
Responsible library:
WPRO
ABSTRACT
BACKGOUND: The present study examined whether a blockade of nitric oxide (NO) synthesis affects the regulation of aquaporin (AQP) water channels in rats subjected to renal ischemia/reperfusion (I/R). METHODS: Renal I/R was experimentally induced by clamping the left renal artery for 60 minutes in rats. The rats were kept for 7 days thereafter, during which they were supplied with tap water containing NG-nitro-L-arginine methyl ester (L-NAME, 100 mg/L). The expression of AQP1-3 was determined in the kidney by Western blot analysis. RESULTS: In renal I/R injury, the expression of AQP2 was significantly decreased. The treatment with L-NAME further diminished the expression of AQP2. Although the expression of either AQP1 or AQP3 was not significantly altered in the kidney subjected to I/R, it was also significantly decreased by the treatment with L-NAME. CONCLUSION: It is suggested that endogenous NO system should play a role in the regulation of AQP water channels in rat kidney subjected to I/R injury.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Renal Artery
/
Reperfusion
/
Blotting, Western
/
Constriction
/
NG-Nitroarginine Methyl Ester
/
Aquaporins
/
Ischemia
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Kidney
/
Nitric Oxide
Limits:
Animals
Language:
En
Journal:
Korean Journal of Nephrology
Year:
2006
Document type:
Article