Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis / 대한배뇨장애요실금학회지
International Neurourology Journal
; : 158-163, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-90691
Responsible library:
WPRO
ABSTRACT
PURPOSE:
The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC).METHODS:
Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction.RESULTS:
In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean+/-standard deviation 1.42+/-0.09 vs. 0.93+/-0.15 and 0.93+/-0.16 vs. 0.29+/-0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05).CONCLUSIONS:
Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Regeneration
/
Tyrosine
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Urinary Bladder
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Protein-Tyrosine Kinases
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RNA, Messenger
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Blotting, Western
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Rats, Sprague-Dawley
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Receptor, ErbB-2
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Cystitis, Interstitial
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Neuregulin-1
Limits:
Animals
Language:
English
Journal:
International Neurourology Journal
Year:
2015
Document type:
Article