Increased p190RhoGEF expression in activated B cells correlates with the induction of the plasma cell differentiation
Experimental & Molecular Medicine
; : 138-148, 2012.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-93416
Responsible library:
WPRO
ABSTRACT
Previously, we demonstrated that the p190 Rho guanine nucleotide exchange factor (p190RhoGEF) was induced following CD40 stimulation of B cells. In this study, we examined whether p190RhoGEF and a downstream effector molecule RhoA are required for B cell differentiation. Expression of p190RhoGEF positively correlated with the expression of surface markers and transcriptional regulators that are characteristic of mature B cells with plasma cell (PC) phenotypes. Moreover, either the overexpression of p190RhoGEF or the expression of a constitutively active RhoA drove cellular differentiation toward PC phenotypes. B cell maturation was abrogated in cells that overexpressed p190RhoGEF and a dominant-negative form of RhoA simultaneously. CD40-mediated maturation events were also abrogated in cells that overexpressed either dominant-negative p190RhoGEF or RhoA. Together, these data provide evidence that p190RhoGEF signaling through RhoA in CD40-activated B cells drives the induction of the PC differentiation.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Plasma Cells
/
B-Lymphocytes
/
Lymphocyte Activation
/
Cell Differentiation
/
Cell Line
/
Cells, Cultured
/
RhoA GTP-Binding Protein
/
Guanine Nucleotide Exchange Factors
/
Mice, Inbred BALB C
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2012
Document type:
Article