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Bilateral superior cervical ganglionectomy attenuates cardiac remodeling and improves cardiac function in pressure-overloaded heart failure mice / 中华心血管病杂志
Chinese Journal of Cardiology ; (12): 345-352, 2021.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-941285
Responsible library: WPRO
ABSTRACT

Objective:

To investigate the effect of bilateral superior cervical ganglionectomy on cardiac remodeling and function in pressure-overloaded heart failure (HF) mice.

Methods:

Pressure-overloaded HF mouse model was produced by severe thoracic aorta banding (sTAB). Bilateral superior cervical ganglionectomy (SCGx) was performed 2 weeks after sTAB. Twenty four 6-week-old male C57BL/6 mice were randomized divided into 4 groups (n=6 each) control group sham sTAB+sham SCGx; denervated group sham sTAB+SCGx; HF group sTAB+sham SCGx; denervated HF group sTAB+SCGx. Cardiac function was measured by echocardiography at week 0, 1, 2, and 4 after sTAB, respectively. All mice were sacrificed at the end of week 4 and heart tissues were harvested. HE and Masson staining were performed. Immunohistochemical staining (IHC) for tyrosine hydroxylase (TH), adrenergic receptor β1 (AR-β1) and CD68 was performed. Western blot was used to determine the protein expression level of TH, B type natriuretic peptide (BNP), and AR-β1.

Results:

Left ventricular ejection fraction (LVEF) declined continuously in HF group. LVEF was similar between denervated HF group and control group at various time points (P>0.05). LVEF was significantly higher in denervated HF group than in HF group at the end of week 4 (P<0.05). HE staining showed that cross sectional cardiomyocyte area was significantly larger in HF group than in control group and denervated HF group (P<0.05), which was similar between denervated HF group and control group (P>0.05). Masson staining showed that fibrosis level was significantly lower in denervated HF group than in HF group (P<0.05). IHC showed that TH+nerves and CD68+ macrophages were significantly increased in HF mice as compared to control mice (P<0.05), whereas this change was abolished in denervated HF group. AR-β1 was significantly down-regulated in HF group compared with control group (P<0.05), which was not affected by denervation (P>0.05). Western blot demonstrated that the expression level of TH and BNP was significantly higher in HF group compared with the control group (P<0.05), whereas this difference was diminished in denervated HF group (P>0.05).

Conclusion:

Bilateral superior cervical ganglionectomy can reduce sympathetic innervation and macrophage infiltration in pressure overloaded failure heart, thus attenuate cardiac remodeling and improve cardiac function.
Subject(s)
Full text: Available Database: WPRIM (Western Pacific) Main subject: Stroke Volume / Ganglionectomy / Cross-Sectional Studies / Ventricular Function, Left / Ventricular Remodeling / Heart Failure / Mice, Inbred C57BL Type of study: Observational study / Prevalence study / Risk factors Limits: Animals Language: Chinese Journal: Chinese Journal of Cardiology Year: 2021 Document type: Article
Full text: Available Database: WPRIM (Western Pacific) Main subject: Stroke Volume / Ganglionectomy / Cross-Sectional Studies / Ventricular Function, Left / Ventricular Remodeling / Heart Failure / Mice, Inbred C57BL Type of study: Observational study / Prevalence study / Risk factors Limits: Animals Language: Chinese Journal: Chinese Journal of Cardiology Year: 2021 Document type: Article
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