Establishment of gas chromatography for content determination of five volatile components in Qingxuan pills / 中国药房

ABSTRACT

OBJECTIVE To estab lish a meth od for the determination of 5 volatile components as menthone ，menthol， pulegone，piperitone and ligustilide in Qingxuan pills. METHODS Seven batches of Qingxuan pills were taken as test samples and determined by gas chromatography. The gas chromatographic column was DB- 5 sillica capillary column ，the carrier gas was nitrogen，the inlet temperature was 200 ℃. The sample size was 1 μL，and the split ratio was 10 ∶ 1. The temperature was programmed（the initial temperature was kept at 100 ℃ for 2 min，and then raised to 220 ℃ at 5 ℃/min for 2 min），and the temperature of the flame ionization detector was 250 ℃. RESULTS The chromatographic peaks of menthone ，menthol，pulegone， piperitone and ligustilide reached the baseline separation ；the linear ranges of the five components were 0.008-0.388，0.010-0.527， 0.006-0.327，0.006-0.312，0.053-2.672 mg/mL（all r＞0.999 0）；the average recoveries were 96.33％（RSD＝1.23％，n＝6）， 96.92％（RSD＝1.38％，n＝6），97.53％（RSD＝1.81％，n＝6），96.80％（RSD＝1.89％，n＝6）and 95.61％（RSD＝0.77％， n＝6）；the contents of the five components were 0.009-0.070，0.040-0.157，0.017-0.150，0.008-0.049 and 0.144-0.932 mg/g， respectively. CONCLUSIONS The gas chromatography method established in this study is simple and accurate ，which can simultaneously determine the contents of five volatile components in Qingxuan pills .