Effect of bortezomib on migration and invasion in cervical carcinoma HeLa cell
Asian Pacific Journal of Tropical Medicine
; (12): 485-488, 2015.
Article
in Zh
| WPRIM
| ID: wpr-951603
Responsible library:
WPRO
ABSTRACT
Objective: To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism. Methods: The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot. Results: MTT assay indicated bortezomib (2.5μM, 5μM, 10μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9. Conclusions: These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2015
Document type:
Article