Profile of Virulence Genes and Rep-PCR Genomic Fingerprinting on Escherichia coli Strains Isolated from Patients with Urinary Tract Infection
Journal of Bacteriology and Virology
; : 1-10, 2003.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-95415
Responsible library:
WPRO
ABSTRACT
The profile of virulence genes and repetitive element sequence-based PCR (rep-PCR) genomic fingerprinting were determined on Escherichia coli strains isolated from patients with urinary tract infection to investigate genetic relatedness and its identification. Thirty nine strains of E. coli were examined genotypically by using the multiplex polymerase chain reaction for the presence of five urovirulence genes; pyelonephritis-associated pili (pap), S. fimbriae (sfa), afimbrial adhesin (afa), cytotoxic necrotizing factor (cnf ), and a-hemolysin (hly). As a result, genotype pap+sfa-afa-cnf -hly- was the most dominant (14 strains 36%). But no urovirulence-genes were detected in 12 strains (31%). On the basis of rep-PCR, the dendrograms generated from REP-PCR and ERIC-PCR revealed that uropathogenic E. coli strains were clustered into non-uropathogenic E. coli ATCC 43894 O157H7 with the degree of similarity 37% and 44%, respectively. On the contrary, BOX-PCR results showed that uropathogenic E. coli strains differed from non-uropathogenic E. coli ATCC 43894 O157H7 with the degree of similarity 37%. According to these findings, REP-PCR and ERIC-PCR were unable to discriminate reliably uropathogenic E. coli from non-uropathogenic E. coli. However, BOX-PCR provided an effective mean of differentiating E. coli strains between uropathogenic and non-uropathogenic.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Urinary Tract
/
Urinary Tract Infections
/
Virulence
/
Polymerase Chain Reaction
/
Dermatoglyphics
/
Escherichia
/
Escherichia coli
/
Multiplex Polymerase Chain Reaction
/
Genotype
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2003
Document type:
Article