Phytohemagglutinins in Philippine plants

ABSTRACT

A survey of several plant seeds which may possess phytohemagglutinating properties indispensable in blood studies and tissue cultures demonstrated that five plant seeds have this property, namely Red Kidney beans, White Kidney Beans, Sigarilyas, Bataw and Soy Bean (local variety)The first two agglutinate blood type A, B, AB and O while sigarilyas A, B and AB; Bataw, A, B and AB while the local soy bean agglutinates only type B and ABBy column chromatography in Ve/Vo studies, RKB, WKB and Sigarilyas had molecular weights of 61,000, 62,000 and 60,000, respectively. Estimated MWs of PHA from RKB, WKB and Sigarilyas compared with the molecular weights of known standard compounds by TLG show RKB, WKB and Sigarilyas had MWs of 62,000, 57,000 and 40,000, respectivelyJaffes Method of Purification has been found most suitable for sigarilyas PHA extractionA comparison of the 1/Rf values of ovalbumin for both column chromatography and Thin Layer Gel Filtration shows that the calibration curves are reliable. However, based on the results of the molecular weight determination or the PHA from the samples, the MWs obtained by column chromatography and TLG differ, the separation by the latter seem more distinct. (Summary)