Influence of meliorated AS-PCR primer for ABO genotyping / 法医学杂志
Journal of Forensic Medicine
; (6): 275-276, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-983198
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To improve the correct rate of ABO genotyping by meliorating AS-PCR primer.@*METHODS@#The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed.@*RESULTS@#The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer.@*CONCLUSION@#The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
ABO Blood-Group System
/
Molecular Sequence Data
/
Base Sequence
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
DNA Primers
/
Alleles
/
Genotype
Limits:
Humans
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2006
Document type:
Article
