Application of MiniFiler kit in forensic DNA testing of low copy number template / 法医学杂志

ABSTRACT

OBJECTIVE@#To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit (MiniFiler) in forensic practice.@*METHODS@#Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer (Applied Biosystems, Foster City, USA).@*RESULTS@#DNA samples as low as 10 pg could be amplified by MiniFiler kit, and the optimal DNA quantity was 40 pg or above.@*CONCLUSION@#MiniFiler kit can be used for analysis of low copy number STR.