Determination of doxepin in whole blood by SPE-LC-MS/MS / 法医学杂志
Journal of Forensic Medicine
; (6): 350-352, 2011.
Article
in Zh
| WPRIM
| ID: wpr-983679
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To develop a method of SPE-LC-MS/MS for the determination of doxepin in whole blood.@*METHODS@#After solid phase extraction, the samples were identified by LC-MS/MS. Positive ion electrospray ionization mode and multiple reaction monitoring (MRM) mode was selected. Amitriptyline was used as internal standard. The m/z of doxepin: 280-->107, 280-->235 and 280-->220. The m/z of amitriptyline: 278-->233. The retaining time of doxepin and amitriptyline were 15.15 and 16.94 min, respectively.@*RESULTS@#The calibration curve was linear among the concentration of doxepin range from 0.005 to 1.00 microg/mL. The linear correlation equation was y = 3.2047x + 0.0339, the correlation coefficient was 0.9996. The detection limit of doxepin was 0.001 microg/mL and average recovery rate was 78.0%-82.9%. The relative standard precision for within-day and between-day were less than 2.55% and 5.90%, respectively.@*CONCLUSION@#The method is effective, simple, reliable and can be used in the determination of doxepin in whole blood.
Full text:
1
Database:
WPRIM
Main subject:
Biomarkers
/
Reproducibility of Results
/
Chromatography, Liquid
/
Sensitivity and Specificity
/
Spectrometry, Mass, Electrospray Ionization
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Doxepin
/
Tandem Mass Spectrometry
/
Solid Phase Extraction
/
Forensic Toxicology
/
Drug Overdose
Limits:
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2011
Document type:
Article