Genetic polymorphisms of 15 X-STR loci in Shandong Han population / 法医学杂志
Journal of Forensic Medicine
; (6): 202-208, 2013.
Article
in Zh
| WPRIM
| ID: wpr-983822
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han population in order to establish the forensic application database.@*METHODS@#The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins (FAM, VIC, NED and TET). The developed multi-PCR was used to investigate 15 X-STR loci (DXS10011, DXS101, GATA 165B12, DXS6795, DXS6800, DXS6801, DXS6803, DXS7132, DXS7133, DXS7423, DXS7424, DXS8377, DXS8378, DXS9898 and HPRTB) selected from the X chromosome of 481 unrelated individuals (295 females and 186 males) in Shandong Han population.@*RESULTS@#Among the 15 X-STR loci, GATA 165B12, DXS6800, DXS6803, DXS7133 and DXS7423 showed moderate polymorphisms, while the rest 10 X-STR loci showed high polymorphisms (PIC > 0.5 and H > 0.5). No shared haplotype was detected among the males in Shandong Han population.@*CONCLUSION@#The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Han population and shows its forensic application.
Full text:
1
Database:
WPRIM
Main subject:
Polymorphism, Genetic
/
China
/
DNA Fingerprinting
/
DNA Primers
/
Microsatellite Repeats
/
Chromosomes, Human, X
/
Asian People
/
Forensic Genetics
/
Multiplex Polymerase Chain Reaction
/
Gene Frequency
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2013
Document type:
Article