Determination of Bloodstain Age by UV Visible Integrating Sphere Reflection Spectrum / 法医学杂志
Journal of Forensic Medicine
; (6): 326-328, 2016.
Article
in Zh
| WPRIM
| ID: wpr-984853
Responsible library:
WPRO
ABSTRACT
OBJECTIVES@#To establish a method for rapid identification of bloodstain age.@*METHODS@#Under laboratory conditions (20 ℃, 25 ℃ and 30 ℃), an integrating sphere ISR-240A was used as a reflection accessory on an UV-2450 UV-vis spectrophotometer, and a standard white board of BaSO₄ was used as reference, the reflection spectrums of bloodstain from human ears' venous blood were measured at regular intervals. The reflection radios R₅₄₁ and R₅₇₇ at a specific wavelength were collected and the value of R₅₄₁/R₅₇₇ was calculated. The linear fitting and regression analysis were done by SPSS 17.0.@*RESULTS@#The results of regression analysis showed that R² of the ratios of bloodstain age to UV visible reflectivity in specific wavelengths were larger than 0.8 within 8 hours and under certain circumstances. The regression equation was established. The bloodstain age had significant correlation with the value of R₅₄₁/R₅₇₇.@*CONCLUSIONS@#The method of inspection is simple, rapid and nondestructive with a good reliability, and can be used to identify the bloodstain age within 8 hours elapsed-time standards under laboratory conditions.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Reference Standards
/
Spectrum Analysis
/
Time Factors
/
Ultraviolet Rays
/
Blood Stains
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Reproducibility of Results
/
Forensic Sciences
Limits:
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2016
Document type:
Article