Research and exploration of salivary biological markers for hepatitis B-related hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 495-503, 2023.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-986159
Responsible library:
WPRO
ABSTRACT
Objective:
To study using isotope-labeled relative and absolute quantitative proteomics methodologies to screen for salivary biological markers as a simple, non-invasive tool for identifying hepatitis B-related HCC at an early stage.Methods:
Saliva samples were collected to extract salivary proteins. Isotope-labeled relative and absolute quantitative proteomics were used to analyze the differentially expressed proteins between the hepatocellular carcinoma (HCC) and non-HCC groups. Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays were used to verify differential proteins and identify markers in liver cancer tissues and saliva. Statistical analysis was used to analyze the diagnostic efficiency of salivary biomarkers.Results:
152 differentially expressed salivary proteins were screened out between the HCC and non-HCC groups. Western blot, immunohistochemistry, and enzyme-linked immunosorbent assays validated that the expressions of α-1-acid glycoprotein 1 (ORM1) and alpha-fetoprotein (AFP) were significantly increased in HCC (P < 0.05). There was a significant correlation between salivary AFP and serum AFP (P < 0.05). HCC was diagnosed when salivary α-1-acid glycoprotein 1 combined with AFP. The area under the receiver operating characteristic curve was 0.8726 (95% confidence interval 0.8104 ~ 0.9347), the sensitivity was 78.3%, and the specificity was 88%.Conclusion:
Salivary AFP and α-1-acid glycoprotein 1 can serve as potential biomarkers for hepatitis B-related hepatocellular carcinoma.
Full text:
Available
Health context:
SDG3 - Health and Well-Being
Health problem:
Target 3.3: End transmission of communicable diseases
Database:
WPRIM (Western Pacific)
Main subject:
Glycoproteins
/
Biomarkers
/
Alpha-Fetoproteins
/
Biomarkers, Tumor
/
ROC Curve
/
Carcinoma, Hepatocellular
/
Hepatitis B
/
Liver Neoplasms
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2023
Document type:
Article