Content determinnation of chlorogenic acid and linarin in Yejuhua Granules / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
; (6): 197-200, 2023.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-989605
Responsible library:
WPRO
ABSTRACT
Objective:
To establish a method for determintation of chlorogenic acid and linarin in Yejuhua granules by HPLC.Methods:
We applied HPLC methods. The Kromasil 100-5 C18 column (250 mm×4.6 mm,5 μm) was used, the mobile phase was acetonitrile-0.4%H 3PO 4 solution (gradient elution), the flow rate was 1.0 ml/min, the dection wavelenghth was 334 nm and the column temperture was 32 ℃.Results:
Chlorogenic acid and buddleoside had good linearity in the ranges of 0.30-1.50 μg ( r2=0.999 1) and 0.12-0.62 μg ( r2=0.999 8), respectively. The average recoveries were 99.70% and 96.67%, with RSD<2%, respectively.Conclusion:
The method is simple, rapid, reliable, efficient, and can be used for determination of chlorogenic acid and buddleoside in Yejuhua Granules.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Journal of Traditional Chinese Medicine
Year:
2023
Document type:
Article