Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes
Rev. bras. reprod. anim
; 40(4): 322-323, Out-Dez. 2016. tab
Article
en Pt
| VETINDEX
| ID: biblio-1492284
Biblioteca responsable:
BR68.1
Ubicación: BR68.1
ABSTRACT
Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.
Palabras clave
Texto completo:
1
Base de datos:
VETINDEX
Asunto principal:
Angiotensinas
/
Ovinos
/
Técnicas de Maduración In Vitro de los Oocitos
Límite:
Animals
Idioma:
Pt
Revista:
Rev. bras. reprod. anim
Año:
2016
Tipo del documento:
Article
/
Congress and conference