Total rupture of Achilles tendon induces inflammatory response and glial activation on the spinal cord of mice
Braz. j. med. biol. res
; 56: e12391, 2023. graf
Artículo
en Inglés
|
LILACS-Express
| LILACS
| ID: biblio-1513881
Biblioteca responsable:
BR1.1
ABSTRACT
Rupture of Achilles tendon is a common accident affecting professional and recreational athletes. Acute and chronic pain are symptoms commonly observed in patients with rupture. However, few studies have investigated whether Achilles tendon rupture is able to promote disorders in the central nervous system (CNS). Therefore, the current study aimed to evaluate nociceptive alterations and inflammatory response in the L5 lumbar segment of Balb/c mice spinal cord after Achilles tendon rupture. We found increased algesia in the paw of the ruptured group on the 7th and 14th days post-tenotomy compared with the control group. This phenomenon was accompanied by overexpression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase-2 (NOS-2) as well as hyperactivation of astrocytes and microglia in nociceptive areas of L5 spinal cord as evidenced by intense GFAP and IBA-1 immunostaining, respectively. Biochemical studies also demonstrated increased levels of nitrite in the L5 spinal cord of tenotomized animals compared with the control group. Thus, we have demonstrated for the first time that total rupture of the Achilles tendon induced inflammatory response and nitrergic and glial activation in the CNS in the L5 spinal cord region.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
LILACS
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2023
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto de Ciências Biológicas, Universidade Federal do Pará/BR
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Instituto de Ciências da Saúde, Universidade Federal do Pará/BR
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Núcleo de Medicina Tropical, Universidade Federal do Pará/BR