Integrin-ß1, not integrin-ß5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
Biol. Res
; 48: 1-8, 2015. graf, tab
Artículo
en Inglés
| LILACS
| ID: biblio-950789
Biblioteca responsable:
CL1.1
ABSTRACT
BACKGROUND:
Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-ß (ß1, ß5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-ß; in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear.RESULTS:
After transfection with integrin-ß1 siRNA or integrin-ß5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µÎµ) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-ß1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-ß5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation.CONCLUSION:
Integrin-ß1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-ß5 is not involved in the osteoblasts response to the tensile strain.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
LILACS
Asunto principal:
Osteoblastos
/
Resistencia a la Tracción
/
Diferenciación Celular
/
Integrina beta1
/
Cadenas beta de Integrinas
/
Matriz Extracelular
Límite:
Animales
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2015
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Academy of Military Medical Sciences/CN
/
Dezhou University/CN
/
Guilin Medical University/CN
/
Logistics College of Chinese People's Armed Police Forces/CN