Pharmacological suppression of rat distal colon chloride secretion requires two blockers.
Acta gastroenterol. latinoam
; 33(3): 119-27, 2003.
Artículo
en Inglés
| BINACIS
| ID: bin-38822
Biblioteca responsable:
AR40.1
ABSTRACT
Rat distal colon epithelium is frequently employed to assess the effect of natural and synthetic chemicals on chloride secretion. Inhibition of chloride secretion is often reported as the loop diuretic-sensitive portion of short-circuit current (Isc). The present work challenges the hypothesis that a loop diuretic alone is able to fully abolish chloride secretion. Isolated mucosa preparations were mounted in an Ussing chamber. The effects on short-circuit current of replacement of normal Ringer by a low (2.5 mmol/L) Cl solution and of blockers of basolateral Na, K, 2 Cl symport (bumetanide), apical Cl channels (diphenylamine-2-carboxylate, DPC), and anion exchange (4-acetamido-4-isothiocyanatostilbene-2,2-disulfonic acid, SITS) alone and combined were assessed. Low Cl reversibly decreased Isc by 76
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Argentina
Base de datos:
BINACIS
Idioma:
Inglés
Revista:
Acta gastroenterol. latinoam
Año:
2003
Tipo del documento:
Artículo