Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies
Biocell
; 27(2): 213-224, Aug. 2003.
Article
en En
| BINACIS
| ID: bin-3990
Biblioteca responsable:
BR1.1
ABSTRACT
CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran-5,6-dione; CG-NQ), a beta-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 microM CG-NQ, the following effects were observed (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen peroxide. Expression of such effects varied according to hepatocyte samples and taken together strongly support an apoptotic action of CG-NQ dependent on reactive oxygen species. (AU)
Texto completo:
1
Colección:
06-national
/
AR
Base de datos:
BINACIS
Asunto principal:
Naftoquinonas
/
Apoptosis
/
Hepatocitos
Límite:
Humans
/
Male
Idioma:
En
Revista:
Biocell
Año:
2003
Tipo del documento:
Article