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High molecular weight components containing N-linked oligosaccharides of ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR
Favoretto, Bruna Cristina; Casabuono, Adriana A. C; Portes Júnior, José Antonio; Jacysyn, Jacqueline F; Couto, Alicia S; Faquim-Mauro, Eliana.
Afiliación
  • Favoretto, Bruna Cristina; Instituto Butantan. Laboratório de Imunopatologia.
  • Casabuono, Adriana A. C; Instituto Butantan. Laboratório de Imunopatologia.
  • Portes Júnior, José Antonio; Instituto Butantan. Laboratório de Imunopatologia.
Mol. Immunol. ; 87: 33-46, 2017.
Article en En | SES-SP, SESSP-IBPROD, SES-SP | ID: but-ib15127
Biblioteca responsable: BR78.1
Ubicación: BR78.1
ABSTRACT
Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molecule and, thus, decreases the T lymphocytes response. Here, we studied the glycoconjugates in PI and the role of C-type lectin receptors (CLRs), DC-SIGN and MR, in the modulation of DCs activity. Our data showed the presence of glycoconjugates with high mannose- and complex-type N-linked oligosaccharide chains and phosphorylcholine residues on PI. In addition, these N-linked glycoconjugates inhibited the DCs maturation induced by LPS. The binding and internalization of PI-Alexa were decreased on DCs previously incubated with mannan, anti-DC-SIGN and/or anti-MR antibodies. In agreement with this, the incubation of DCs with mannan, anti-DC-SIGN and/or anti-MR antibodies abolished the down-modulatory effect of PI on these cells. It was also observed that the blockage of CLRs, DC-SIGN and MR on DCs reverted the inhibitory effect of PI in in vitro T cells proliferation. Therefore, our data show the involvement of DC-SIGN and MR in the recognition and consequent modulatory effect of N-glycosylated components of PI on DCs.
Texto completo: 1 Colección: 06-national / BR Base de datos: SES-SP / SESSP-IBPROD Idioma: En Revista: Mol. Immunol. Año: 2017 Tipo del documento: Article
Texto completo: 1 Colección: 06-national / BR Base de datos: SES-SP / SESSP-IBPROD Idioma: En Revista: Mol. Immunol. Año: 2017 Tipo del documento: Article