Protective effects of aloin on asthmatic mice by activating Nrf2/HO-1 pathway and inhibiting TGF-B/Smad2/3 pathway
Allergol. immunopatol
; 51(4): 10-18, 2023. graf
Artículo
en Inglés
| IBECS
| ID: ibc-222630
Biblioteca responsable:
ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
Background:
Asthma is a severe chronic respiratory disease affecting all age groups with increasing prevalence. Anti-inflammatory strategies are promising options for the treatment of asthma. Although the inhibitory effect of aloin on inflammation has been demonstrated in various diseases, its effect on asthma remains unknown.Methods:
A mice asthma model was established by treating with ovalbumin (OVA). The effects and mechanism of aloin on the OVA-treated mice were determined by enzyme-linked--immunosorbent serologic assay, biochemical examination, hematoxylin and eosin and Masson's staining, and Western blot assay.Results:
OVA treatment in mice significantly increased the number of total cells, neutrophils, eosinophils, and macrophages and the concentration of interleukin (IL)-4, IL-5, and IL-13, which were attenuated with the administration of aloin. The content of malondialdehyde was enhanced in OVA-treated mice, with the decreased levels of superoxide dismutase and glutathione, which were reversed with aloin treatment. Aloin treatment reduced the airway resistance of OVA-induced mice. The inflammatory cell infiltration around small airways was accompanied by the thickening and contraction of bronchial walls and pulmonary collagen deposition in OVA-treated mice; however, these conditions were ameliorated with aloin treatment. Mechanically, aloin upregulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2)heme oxygenase 1 (HO-1) pathway but inhibited the level of transforming growth factor betaSMAD2/3 genes (TGF-β/Smad2/3) axis in OVA-induced mice.Conclusion:
Aloin treatment lessened airway hyperresponsiveness, airway remodeling, inflammation, and oxidative stress in OVA-treated mice, and was closely related to the activation of Nrf2/HO-1 pathway and the weakening of TGF-β/Smad2/3 pathway (AU)
Texto completo:
Disponible
Colección:
Bases de datos nacionales
/
España
Base de datos:
IBECS
Asunto principal:
Asma
/
Factor de Crecimiento Transformador beta
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Emodina
/
Factor de Transcripción NF-E2
Límite:
Animales
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Humanos
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Masculino
Idioma:
Inglés
Revista:
Allergol. immunopatol
Año:
2023
Tipo del documento:
Artículo
Institución/País de afiliación:
The Second Affiliated Clinical Hospital of Harbin Medical University/China
/
The Second Affiliated Hospital of Harbin Medical University/China