Adiposity and serum parameters in hamsters fed energy restricted diets supplemented or not with trans-10, cis-12 conjugated linoleic acid

ABSTRACT

Numerous studies have demonstrated that conjugated linoleic acid (CLA) modulatesbody composition, reducing body fat accumulation in various mammalianspecies. However, very few studies have been carried out to assess the effect of CLAon previously stored body fat. The aim of the present work was to analyse the effectivenessof trans-10,cis-12 CLA in improving alterations produced by high-fat feedingin body fat and serum parameters when it was included in an energy-restricteddiet. For this purpose male Syrian Golden hamsters were fed on high-fat diet for 7weeks in order to increase their body fat content, and a further 25% energy-restricteddiet supplemented or not with 0.5% trans-10,cis-12 CLA for 3 weeks. Adipose tissues,liver and gastrocnemious muscles were dissected and weighed. Adipocyte diameterand number were assessed in epididymal adipose tissue. Total cholesterol, triacylglycerols,non-esterified fatty acids and glucose were measured in serum. Threeweeks of energy restriction resulted in a reduction in body weight and white adiposetissue size in all anatomical locations, without changes in liver and gastrocnemiousmuscle weights. Epididymal adipocyte size was reduced, but total adipocyte numberremained unchanged. Serum cholesterol, triacylglycerols and glucose were significantlyreduced. No differences were observed between the restricted groups (controland CLA supplemented). In conclusion, under our experimental conditions, theaddition of trans-10,cis-12 CLA to the diet does not increase the benefits producedby energy restriction (AU)

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