Hsp65 PCR-restriction enzyme analysis (PRA) for identification of mycobacteria in the clinical laboratory
Rev. Inst. Med. Trop. Säo Paulo
; Rev. Inst. Med. Trop. Säo Paulo;43(1): 25-28, Jan.-Feb. 2001. ilus, tab
Article
en En
| LILACS
| ID: lil-285679
Biblioteca responsable:
BR1.1
RESUMO
More than 70 species of mycobacteria have been defined, and some can cause disease in humans, especially in immunocompromised patients. Species identification in most clinical laboratories is based on phenotypic characteristics and biochemical tests and final results are obtained only after two to four weeks. Quick identification methods, by reducing time for diagnosis, could expedite institution of specific treatment, increasing chances of success. PCR restriction-enzyme analysis (PRA) of the hsp65 gene was used as a rapid method for identification of 103 clinical isolates. Band patterns were interpreted by comparison with published tables and patterns available at an Internet site (http//www.hospvd.ch8005). Concordant results of PRA and biochemical identification were obtained in 76 out of 83 isolates (91.5 percent). Results from 20 isolates could not be compared due to inconclusive PRA or biochemical identification. The results of this work showed that PRA could improve identification of mycobacteria in a routine setting because it is accurate, fast, and cheaper than conventional phenotypic identification
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Enzimas de Restricción del ADN
/
Reacción en Cadena de la Polimerasa
/
Chaperoninas
/
Mycobacterium
/
Infecciones por Mycobacterium
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
MEDICINA TROPICAL
Año:
2001
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil