Respuesta humoral específica en ratones Balb/c inoculados con una librería genómica de expresión de Trypanosoma cruzi / Specific humoral response in Balb/c mice inoculated with a genome library of expression of Trypanosoma cruzi
Rev. cuba. med. trop
; 51(1): 20-25, ene.-abr. 1999.
Article
en Es
| LILACS
| ID: lil-333545
Biblioteca responsable:
BR1.1
RESUMO
A genomic expression library of Trypanosoma cruzi (T. cruzi) was made using plasmid pcDNA3 as a vector, with which male mice from the Balb/c isogenic line were intramuscullary inoculated. It was used a positive control group that was administered soluble antigens of T. cruzi. Other 2 groups received genomic and plasmid DNA, respectively. One group was not immunized. Weekly blood samples were obtained from all the animals until the fourth week and 2 weeks after reimmunization to study the response of specific antibodies against the microorganism antigens by an indirect immunoenzymatic assay (ELISA). It was observed a significant increase of specific antibodies in the animals reimmunized with 50 micrograms of the library, as well as in the group immunized with soluble antigens of T. cruzi.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Trypanosoma cruzi
/
Anticuerpos Antiprotozoarios
/
Biblioteca Genómica
/
ADN Protozoario
Límite:
Animals
Idioma:
Es
Revista:
Rev. cuba. med. trop
Asunto de la revista:
MEDICINA TROPICAL
Año:
1999
Tipo del documento:
Article
País de afiliación:
Cuba
Pais de publicación:
Cuba