Cholestasis in a murine experimental model: lesions include hepatocyte ischemic necrosis
Rev. Hosp. Clin. Fac. Med. Univ. Säo Paulo
; 58(1): 27-32, Jan.-Feb. 2003. ilus
Article
en En
| LILACS
| ID: lil-335227
Biblioteca responsable:
BR1.1
ABSTRACT
OBJECTIVE:
To establish a murine experimental model of bile duct obstruction that would enable controlled observations of the acute and subacute phases of cholestasis.METHODOLOGY:
Adult male isogenic BALB/c mice underwent a bile duct ligation (22 animals) or a sham operation (10 animals). Fifteen days after surgery, or immediately after the animal's death, macroscopic findings were noted and histological study of the liver, biliary tree, and pancreas was performed (hematoxylin-eosin and Masson trichromic staining).RESULTS:
Beginning 24 hours after surgery, all animals from the bile duct ligation group presented progressive generalized malaise. All animals presented jaundice in the parietal and visceral peritoneum, turgid and enlarged liver, and accentuated dilatation of gallbladder and common bile duct. Microscopic findings included marked dilatation and proliferation of bile ducts with accentuated collagen deposits, frequent areas of ischemic necrosis, hepatic microabscesses, and purulent cholangitis. Animals from the sham operation group presented no alterations.CONCLUSION:
We established a murine experimental model of induced cholestasis, which made it possible to study acute and subacute tissue lesions. Our data suggests that in cholestasis, hepatic functional ischemia plays an important role in inducing hepatic lesions, and it also suggests that the infectious process is an important factor in morbidity and mortality
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Conductos Biliares
/
Colestasis
/
Hepatocitos
/
Modelos Animales de Enfermedad
/
Isquemia
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Rev. Hosp. Clin. Fac. Med. Univ. Säo Paulo
Asunto de la revista:
MEDICINA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil