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Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations
Cestari, Marta Margarete; Lemos, Priscilla Maria M; Ribeiro, Ciro Alberto de Oliveira; Costa, João Ricardo M. Alves; Pelletier, Emilien; Ferraro, Marcos V. M; Mantovani, Mário Sérgio; Fenocchio, Alberto Sergio.
Afiliación
  • Cestari, Marta Margarete; Universidade Federal do Paraná. Departamento de Genética. Curitiba. BR
  • Lemos, Priscilla Maria M; Universidade Federal do Paraná. Departamento de Genética. Curitiba. BR
  • Ribeiro, Ciro Alberto de Oliveira; Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba. BR
  • Costa, João Ricardo M. Alves; Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba. BR
  • Pelletier, Emilien; Institut des Sciences de la Mer de Rimouski. Quebec. CA
  • Ferraro, Marcos V. M; Universidade Federal do Paraná. Departamento de Genética. Curitiba. BR
  • Mantovani, Mário Sérgio; Universidade Estadual de Londrina. Departamento de Biologia Geral. Londrina. BR
  • Fenocchio, Alberto Sergio; Universidad Nacional de Misiones. Departamento de Genética. Posadas. AR
Genet. mol. biol ; Genet. mol. biol;27(2): 270-274, Jun. 2004. ilus, tab
Article en En | LILACS | ID: lil-362899
Biblioteca responsable: BR26.1
ABSTRACT
The effects of clastogenic or mutagenic agents have rarely been studied in neotropical fish species exposed to contaminated water. In this study, the genetic damage caused by lead in the widely distributed South American fish, Hoplias malabaricus, was assessed using the comet (SCGE) assay and by testing for chromosomal aberrations. Eighteen specimens were acclimatized to laboratory conditions and then chronically exposed to contaminated food by feeding prey (Cyprinus sp.) injected intraperitoneally with doses of inorganic lead adjusted to give a contamination level of 21 mg of Pb2+.g-1 net weight of H. malabaricus. Three fish were sampled for chromosomal analysis after four doses (18 days) and another three after eight doses (41 days) of lead and the results then compared with three untreated controls kept under lead-free conditions. An additional six treated fish and three controls were sampled for the comet assay after 13 doses (64 days). Exposure to lead significantly increased the frequency of chromosomal aberrations and the frequency of tailed cell nuclei, the latter indicating DNA damage. These results show that H. malabaricus is a useful biological model for screening the clastogenic effects of lead and possibly other xenobiotics. The genetic damage seen here illustrates the need to investigate the potential effects of heavy metals on fish species in South America.
Asunto(s)
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Daño del ADN / Aberraciones Cromosómicas / Peces Límite: Animals Idioma: En Revista: Genet. mol. biol Asunto de la revista: GENETICA Año: 2004 Tipo del documento: Article / Project document País de afiliación: Argentina / Brasil / Canadá Pais de publicación: Brasil
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Daño del ADN / Aberraciones Cromosómicas / Peces Límite: Animals Idioma: En Revista: Genet. mol. biol Asunto de la revista: GENETICA Año: 2004 Tipo del documento: Article / Project document País de afiliación: Argentina / Brasil / Canadá Pais de publicación: Brasil