Effect of D-alpha-tocopherol on tubular nephron acidification by rats with induced diabetes mellitus
Braz. j. med. biol. res
; 38(7)July 2005. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-403859
Biblioteca responsable:
BR1.1
RESUMO
The objective of the present study was to determine if treatment of diabetic rats with D-alpha-tocopherol could prevent the changes in glomerular and tubular function commonly observed in this disease. Sixty male Wistar rats divided into four groups were studied control (C), control treated with D-alpha-tocopherol (C + T), diabetic (D), and diabetic treated with D-alpha-tocopherol (D + T). Treatment with D-alpha-tocopherol (40 mg/kg every other day, ip) was started three days after diabetes induction with streptozotocin (60 mg/kg, ip). Renal function studies and microperfusion measurements were performed 30 days after diabetes induction and the kidneys were removed for morphometric analyses. Data are reported as means ± SEM. Glomerular filtration rate increased in D rats but decreased in D + T rats (C 6.43 ± 0.21; D 7.74 ± 0.45; D + T 3.86 ± 0.18 ml min-1 kg-1). Alterations of tubular acidification observed in bicarbonate absorption flux (JHCO3) and in acidification half-time (t/2) in group D were reversed in group D + T (JHCO3, C 2.30 ± 0.10; D 3.28 ± 0.22; D + T 1.87 ± 0.08 nmol cm-2 s-1; t/2, C 4.75 ± 0.20; D 3.52 ± 0.15; D + T 5.92 ± 0.19 s). Glomerular area was significantly increased in D, while D + T rats exhibited values similar to C, suggesting that the vitamin prevented the hypertrophic effect of hyperglycemia (C 8334.21 ± 112.05; D 10,217.55 ± 100.66; D + T 8478.21 ± 119.81æm²). These results suggest that D-alpha-tocopherol is able to protect rats, at least in part, from the harmful effects of diabetes on renal function.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
LILACS
Asunto principal:
Acidosis Tubular Renal
/
Alfa-Tocoferol
/
Diabetes Mellitus Experimental
/
Nefropatías Diabéticas
/
Nefronas
/
Antioxidantes
Límite:
Animales
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2005
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de São Paulo/BR
/
Universidade de São Paulo/BR