Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa
Genet. mol. res. (Online)
; Genet. mol. res. (Online);5(4): 851-855, 2006. ilus
Article
en En
| LILACS
| ID: lil-482073
Biblioteca responsable:
BR1.1
ABSTRACT
DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yield from the hyphae of Crinipellis perniciosa, which causes witches' broom disease in cacao, from three other filamentous fungi, Lentinus edodes, Agaricus blazei, Trichoderma stromaticum, and from the yeast S. cerevisiae. Genomic DNA was suitable for PCR of specific actin primers of C. perniciosa, allowing it to be differentiated from fungal contaminants, including its natural competitor, T. stromaticum.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
ADN de Hongos
/
Técnicas de Tipificación Micológica
/
Genoma Fúngico
/
Agaricales
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil