Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L. )
Electron. j. biotechnol
; 14(6): 6-6, Nov. 2011. ilus, tab
Article
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| LILACS
| ID: lil-640523
Biblioteca responsable:
CL1.1
ABSTRACT
Background:
Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods.Results:
Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66 percent). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming.Conclusions:
In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Arachis
/
Enfermedades de las Plantas
/
Genes de Plantas
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Inmunidad Innata
Idioma:
En
Revista:
Electron. j. biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2011
Tipo del documento:
Article
/
Project document
País de afiliación:
China