Development of a subcutaneous endometriosis rat model
Acta cir. bras
; Acta cir. bras;30(1): 6-12, 01/2015. tab, graf
Article
en En
| LILACS
| ID: lil-735705
Biblioteca responsable:
BR1.1
ABSTRACT
PURPOSE:
To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs.METHODS:
Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight 29.1 ± 6.7mg%, relative dry weight 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14.RESULTS:
The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups.CONCLUSION:
The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs. .Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Enfermedades del Tejido Conjuntivo
/
Tejido Subcutáneo
/
Modelos Animales de Enfermedad
/
Endometriosis
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Asunto de la revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2015
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil