Development and characterization of DNA sequence OmyP9 associated with the sex chromosomes in rainbow trout.
Heredity (Edinb)
; 86(Pt 4): 412-9, 2001 Apr.
Article
en En
| MEDLINE
| ID: mdl-11520341
ABSTRACT
This work describes the construction and characterization of a sequence characterized amplified DNA region (SCAR DNA), designated OmyP9, that was derived from a RAPD marker associated with the sex chromosomes in rainbow trout. A RsaI restriction fragment length polymorphism in OmyP9 identifies variants A, B and C. We found six OmyP9 variant phenotypes - A, B, C, AB, BC and ABC, in 186 individuals of seven different rainbow trout strains. The patterns of inheritance of OmyP9 in 139 fingerlings from 10 crosses of three strains of rainbow trout were studied. The males had a greater representation of the A variant (93.3%) suggesting an association with the Y chromosome. All male fingerlings analysed inherited the A variant from their male parents. These results support the hypothesis that OmyP9 is located on the sex chromosomes of rainbow trout, and that for the males studied the A variant is located on the Y chromosome in a region close to sex determinants and/or in a sector where the genetic recombination between X and Y is restricted. The present evidence also supports our previous hypothesis that OmyP9 is organized as a tandem repeated sequence in the sex chromosomes of rainbow trout. We feel that the OmyP9 RsaI marker can be used for sex identification in crosses where it is possible to determine the phenotype of the parents.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromosomas Sexuales
/
ADN
/
Oncorhynchus mykiss
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Heredity (Edinb)
Año:
2001
Tipo del documento:
Article
País de afiliación:
Chile