[Ability of acceptance for bacteriophages during verotoxin production by bacilli of the Enterobacteriaceae family]. / Zdolnosc akceptacji bakteriofagów warunkujacych produkcje verotoksyn przez paleczki z rodziny Enterobacteriaceae.
Med Dosw Mikrobiol
; 53(3): 269-76, 2001.
Article
en Pl
| MEDLINE
| ID: mdl-11785184
Lysogenised verotoxigenic strains are the source of structural genes of verocytotoxins (stx-1 and stx-2) for the others intestinal bacili. The aim of the study was to estimate the ability of transfer of bacteriophages induced with UV irradiation from reference verotoxigenic strains of E. coli O157:H7 (CB571 and EDL933) into 125 wild-strains of bacili of Enterobacteriaceae family. None of tested recipient strains showed the production of cytotoxin on Vero and HeLa cell lines, what was acknowledged as the lack of six genes. Contrary to the laboratory strain of E. coli C600 none of 125 tested recipient strains accepted the phages. Obtained lysogenised laboratory strains of E. coli C600/CB571 and E. coli C600/EDL933, besides of the ability to produce verotoxins (with the presence of stx-1 and stx-2 genes), did not differ phenotypically and genotypically from parent strain of E. coli C600. The estimation of the ability to transfer of phages carried stx-1 and/or stx-2 genes was impossible because of too small number of tested wild strain of bacili or because of really low frequency of acceptation of phages by wild strains of intestinal bacili.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacteriófagos
/
Toxinas Shiga
/
Escherichia coli
Límite:
Animals
/
Humans
Idioma:
Pl
Revista:
Med Dosw Mikrobiol
Año:
2001
Tipo del documento:
Article
Pais de publicación:
Polonia