[Effect of curcumin on expression of human low density lipoprotein receptors in Xenopus Laevis oocytes].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 25(5): 432-5, 2005 May.
Article
en Zh
| MEDLINE
| ID: mdl-15957838
ABSTRACT
OBJECTIVE:
To investigate the molecular mechanism of curcumin in reducing blood lipids by establishing gene expression system of human low density lipoprotein receptors (LDL-R) in Xenopus Laevis oocytes (XLO).METHODS:
The expression of LDL-R on cytomembrane was determined using immuno-fluorescent, ligand-fluorescent and immune colloidal gold techniques after human LDL-R containing p3.7 LDL plasmid was led into nucleus. And the expression of LDL-R gene in XLO was quantitatively determined by ELISA after being interfered with different concentrations of curcumin.RESULTS:
The human LDL-R gene could be expressed on XLO, which could be significantly enhanced by curcumin in a dose-dependent manner. Conclusion One of the paths of curcumin in reducing blood lipids and anti-atherosclerosis was improving LDL-R gene expression and increasing the LDL-cholesterol absorption of cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oocitos
/
Receptores de LDL
/
Curcumina
Límite:
Animals
/
Female
/
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2005
Tipo del documento:
Article