Promoter analysis of human CC chemokine CCL23 gene in U937 monocytoid cells.
Biochim Biophys Acta
; 1769(3): 204-8, 2007 Mar.
Article
en En
| MEDLINE
| ID: mdl-17368823
ABSTRACT
Expression of CCL23 is induced by external stimuli including PMA in monocytes, but its transcriptional regulation has not been studied to date. Serial deletion analysis of its 5' flanking region revealed that the region -293 to +31 was important for induction by PMA. Cis-acting elements at the -269/-264 (NFAT site), -167/-159 (NF-kappaB site), and -51/-43 (AP-1 site) positions were identified as the critical sites for the CCL23 expression in U937 cells. We demonstrated the binding of the transcription factors to the consensus sites. Specific inhibitors for signal pathways reduced PMA-induced expression of CCL23, confirming involvement of these transcription factors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Monocitos
/
Regulación de la Expresión Génica
/
Regiones Promotoras Genéticas
/
Quimiocinas CC
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2007
Tipo del documento:
Article