The Kruppel-associated box repressor domain can trigger de novo promoter methylation during mouse early embryogenesis.
J Biol Chem
; 282(47): 34535-41, 2007 Nov 23.
Article
en En
| MEDLINE
| ID: mdl-17893143
ABSTRACT
The Krüppel-associated box (KRAB) domain is a transcriptional repression module responsible for the DNA binding-dependent gene silencing activity of hundreds of vertebrate zinc finger proteins. We previously exploited KRAB-mediated repression within the context of a tet repressor-KRAB fusion protein and of lentiviral vectors to create a method of external gene control. We demonstrated that with this system transcriptional silencing was fully reversible in cell culture as well as in vivo. Here we reveal that, in sharp contrast, KRAB-mediated repression results in irreversible gene silencing through promoter DNA methylation if it acts during the first few days of mouse development.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
/
Transcripción Genética
/
Proteínas Nucleares
/
Proteínas Portadoras
/
Regiones Promotoras Genéticas
/
Metilación de ADN
/
Desarrollo Embrionario
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
2007
Tipo del documento:
Article
País de afiliación:
Suiza