Thyroid hormones affect the level and activity of nitric oxide synthase in rat cerebral cortex during postnatal development.
Neurochem Res
; 33(3): 569-78, 2008 Mar.
Article
en En
| MEDLINE
| ID: mdl-17940896
ABSTRACT
The effects of thyroid hormones (TH) on the enzyme level and activity of neuronal nitric oxide synthase (nNOS) were studied in the rat cerebral cortex during postnatal life. As revealed by arginine/citrulline conversion assay and Western blot analysis of the homogenate of the parietal cortex T4 significantly increased nNOS activity and nNOS protein level to 153 +/- 25% and to 178 +/- 20%, respectively. In contrast, 6-n-propyl-2-thyouracil (PTU) decreased nNOS activity and nNOS level to 45 +/- 10% and to 19 +/- 4%, respectively. The number of nNOS-immunoreactive neurons did not change after either T4 or PTU treatment, however, following T4 administration the percentage of intensively immunoreactive neurons increased to 85 +/- 3% compared to control (65 +/- 6%), whereas it decreased to 49 +/- 2% after PTU treatment. Our findings indicate that abnormal TH levels differentially regulate the activity and the level of nNOS and suggest a cross-talk between the TH and NO signaling pathway in the developing cerebral cortex of rats.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hormonas Tiroideas
/
Corteza Cerebral
/
Óxido Nítrico Sintasa de Tipo I
Límite:
Animals
Idioma:
En
Revista:
Neurochem Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Hungria