[Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells]. / Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzhei Saccharomyces cerevisiae.

ABSTRACT

The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.