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[Construction of recombinant CHO cell strain for high expression of HBsAg].
Yi, Yao; Guo, Min-Zhuo; Tian, Rui-Guang; Su, Qiu-Dong; Lu, Xue-Xin; Qiu, Feng; Zhou, Wen-Ting; Jia, Zhi-Yuan; Bi, Sheng-Li.
Afiliación
  • Yi Y; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Guo MZ; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Tian RG; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Su QD; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Lu XX; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Qiu F; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Zhou WT; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Jia ZY; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
  • Bi SL; National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi ; 27(4): 289-91, 2013 Aug.
Article en Zh | MEDLINE | ID: mdl-24579478
ABSTRACT

OBJECTIVE:

To overexpress hepatitis B virus S gene in CHO cells cultured in serum-free media.

METHOD:

Plasmid was constructed by cloning of HBV S gene and then it was transfected into CHO cells. After cell screen, the positive clones were identified and isolated into a serum-free media followed by the serological and morphological characterization of the expression product.

RESULT:

CHO cell strains which can express HBsAg efficiently and stably were obtained. Spherical and filamentous HBsAg could be detected under electronic microscope. The titer of the expression product was up to 15000.

CONCLUSION:

Serum-free media cultured CHO cell strain for overexpression of HBsAg was successfully constructed and the expression product was high antigenic.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Expresión Génica / Antígenos de Superficie de la Hepatitis B Límite: Animals Idioma: Zh Revista: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi Asunto de la revista: VIROLOGIA Año: 2013 Tipo del documento: Article País de afiliación: China
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Expresión Génica / Antígenos de Superficie de la Hepatitis B Límite: Animals Idioma: Zh Revista: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi Asunto de la revista: VIROLOGIA Año: 2013 Tipo del documento: Article País de afiliación: China