A rapid HPLC method for determination of zolpidem and its degradation product in tablets using a monolithic column.
J Chromatogr Sci
; 53(5): 807-10, 2015.
Article
en En
| MEDLINE
| ID: mdl-25754693
ABSTRACT
A simple, accurate reverse phase high-performance liquid chromatographic method, utilizing a monolithic silica column, for determination of zolpidem hemitartrate and its degradation product in tablet dosage form was developed. Analysis was achieved on the monolithic, C18 (100 mm, 3.9 mm) column, in isocratic mode with acetonitrile-NaH2PO4 (pH 7.0; 0.01 M; 3565, v/v) as mobile phase and a flow rate of 2.5 mL/min at room temperature with UV detection at 245 nm. Diazepam was applied as an internal standard. The retention time of zolpidem and its degradation product was 2.14 and 1.89, respectively. Calibration curve was linear in the range of 0.12-5 µg/mL and the recovery values were found to be 97-101%. The limit of quantitation was determined 0.12 µg/mL. The relative standard deviation values of intraday and interday studies were calculated as 0.13-1.1% and 0.54-1.3%, respectively.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piridinas
/
Cromatografía Líquida de Alta Presión
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
J Chromatogr Sci
Año:
2015
Tipo del documento:
Article
País de afiliación:
Irán