New approaches for the standardization and validation of a real-time qPCR assay using TaqMan probes for quantification of yellow fever virus on clinical samples with high quality parameters.
Hum Vaccin Immunother
; 11(7): 1865-71, 2015.
Article
en En
| MEDLINE
| ID: mdl-26011746
Palabras clave
17DD, strain used to yellow fever vaccine; ANVISA, Brazilian Health Surveillance Agency; C, capsid protein; CV, coefficient of variation; Ct, cycle threshold; DENV, dengue virus; DL, detection limit; DNA, deoxyribonucleic acid; E, envelope protein; ELISA, enzyme-linked immunosorbent assay; EXO IPC, exogenous internal positive control; FDA, food and drug administration agency; JEV, japanese encephalitis virus; MOI, multiplicity of infection; MV, measles virus; MuV, mumps virus; NS, nonstructural protein; NS5, protein of the viral polyprotein, it is the largest and the most highly conserved among the flaviviral proteins; PCR, polymerase chain reaction; PFU, plaque former unit; QL, quantification limit; RNA, ribonucleic acid; RNAse P, human constitutive gene; RT-qPCR; RT-qPCR, reverse transcriptase quantitative polymerase chain reaction; WNV, West Nile Virus; YF, yellow fever; YFV, yellow fever virus; molecular diagnosis; prM/M, membrane protein; viral load; viral vaccines; yellow fever virus
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fiebre Amarilla
/
Virus de la Fiebre Amarilla
/
Vacuna contra la Fiebre Amarilla
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Hum Vaccin Immunother
Año:
2015
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos