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Sequence variation and bioinformatics analysis of Toxoplasma gondii GRA16 Gene.
Hu, L Y; Zhang, N Z; Gao, Q; Chen, J; Wen, F L; Wang, S K; Zhu, X Q.
Afiliación
  • Hu LY; College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China.
  • Zhang NZ; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
  • Gao Q; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
  • Chen J; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
  • Wen FL; College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China.
  • Wang SK; College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China.
  • Zhu XQ; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
Trop Biomed ; 32(3): 557-62, 2015 Sep.
Article en En | MEDLINE | ID: mdl-26695219
Toxoplasmosis is caused by the intracellular protozoan Toxoplasma gondii. It is anopportunistic zoonosis in warm-blooded animals and humans, with a worldwide distribution. Toxoplasma gondii dense granule protein 16 (TgGRA16) can modulate some functions in host cells and is considered a significant virulent factor of the parasite. The present study reports sequence variation in TgGRA16 gene among T. gondii strains from different hosts and geographical locations, and the construction of phylogenetic relationships of these T. gondii strains based on sequences of TgGRA16, and analysis of B cell epitopes in TgGRA16. Our results showed that all TgGRA16 gene sequences were 1518 bp and the C+G contents ranged from 52.17% to 52.59%. Sequence variation in the TgGRA16 gene was 0-1.51%. Phylogenetic analysis revealed that TgGRA16 gene sequence could not be used to differentiate the different T. gondii genotypes. Six B cell epitopes were predicted in TgGRA16. These results indicated that TgGRA16 gene is not an ideal marker for studying genetic relationships of T. gondii isolates, but may represent a good vaccine candidate against toxoplasmosis.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Variación Genética / Proteínas Protozoarias / Factores de Virulencia Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Trop Biomed Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Malasia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Variación Genética / Proteínas Protozoarias / Factores de Virulencia Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Trop Biomed Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: Malasia