A high-throughput microtiter plate assay for the discovery of active and enantioselective amino alcohol-specific transaminases.

Zhang, Jian-Dong; Wu, Hua-Lei; Meng, Tong; Zhang, Chao-Feng; Fan, Xiao-Jun; Chang, Hong-Hong; Wei, Wen-Long

Zhang, Jian-Dong; Wu, Hua-Lei; Meng, Tong; Zhang, Chao-Feng; Fan, Xiao-Jun; Chang, Hong-Hong; Wei, Wen-Long.

Afiliación

Zhang JD; Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China. Electronic address: zhangjiandong@tyut.edu.cn.
Wu HL; Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China.
Meng T; Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China.
Zhang CF; Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China. Electronic address: zhangchaofeng@tyut.edu.cn.
Fan XJ; Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China.
Chang HH; Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China.
Wei WL; Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, PR China.

Anal Biochem ; 518: 94-101, 2017 Feb 01.

Article en En | MEDLINE | ID: mdl-27899283

Asunto(s)

Amino Alcoholes/química; Proteínas Bacterianas/química; Propanolaminas/química; Pseudomonas putida/enzimología; Transaminasas/química; Amino Alcoholes/metabolismo; Proteínas Bacterianas/metabolismo; Propanolaminas/metabolismo; Especificidad por Sustrato/fisiología; Transaminasas/metabolismo

Palabras clave

Chiral vicinal amino alcohol; Enantioselective; High-throughput screening; Microtiter plate assay; Transaminase

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Propanolaminas / Proteínas Bacterianas / Pseudomonas putida / Amino Alcoholes / Transaminasas Idioma: En Revista: Anal Biochem Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos

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